Journal: Advanced Science
Article Title: Photo‐Empowered Macrophage‐Based Drug Delivery System Overcomes Motility Suppression and Significantly Enhances Deep Tumor Drug Delivery
doi: 10.1002/advs.202515349
Figure Lengend Snippet: A) Schematic diagram of the experimental design for assessing the motility of Raw264.7 cells after metabolic intervention or hypoxia induction in vitro using living cell imaging technology. B) Representative images and migration tracks of Raw264.7 cells under different treatments. C) Average intracellular ATP content ( n = 3), and D) cell migration velocity under different treatment conditions ( n = 5). E) CLSM images of hypoxia levels in 4T1 and MCF‐7 cells marked with hypoxia probes. F) CLSM images of HIF‐1α expression in 4T1 and MCF‐7 cells labeled by immunofluorescence staining. G,H) Semi‐quantitative analysis of hypoxia levels and HIF‐1α expression by fluorescence intensity ( n = 3). Data are presented as mean ± SD. Statistical significance was calculated via one‐way ANOVA with Tukey’ post hoc test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Article Snippet: Mouse breast carcinoma cells 4T1 (RRID: CVCL_0125), human breast carcinoma cells MCF‐7 (RRID: CVCL_0031), and mouse monocyte‐macrophage cells RAW264.7 (RRID: CVCL_0493) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) in February 2023.
Techniques: In Vitro, Imaging, Migration, Expressing, Labeling, Immunofluorescence, Staining, Fluorescence